LEI 13798 PDF

DNAzymes, which are sequences of DNA with catalytic activity, have been demonstrated as a potential platform for sensing a wide range of metal ions. Despite their significant promise, cellular sensing using DNAzymes has however been difficult, mainly because of the "always-on" mode of first-generation DNAzyme sensors. To overcome this limitation, a photoactivatable or photocaged DNAzyme was designed and synthesized, and its application in sensing Zn II in living cells was demonstrated. In this design, the adenosine ribonucleotide at the scissile position of the DNAzyme was replaced by 2'-O-nitrobenzyl adenosine, rendering the DNAzyme inactive and thus allowing its delivery into cells intact, protected from nonspecific degradation within cells. Irradiation at nm restored DNAzyme activity, thus allowing the temporal control over the sensing activity of the DNAzyme for metal ions.

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DNAzymes, which are sequences of DNA with catalytic activity, have been demonstrated as a potential platform for sensing a wide range of metal ions. Despite their significant promise, cellular sensing using DNAzymes has however been difficult, mainly because of the "always-on" mode of first-generation DNAzyme sensors. To overcome this limitation, a photoactivatable or photocaged DNAzyme was designed and synthesized, and its application in sensing Zn II in living cells was demonstrated.

In this design, the adenosine ribonucleotide at the scissile position of the DNAzyme was replaced by 2'-O-nitrobenzyl adenosine, rendering the DNAzyme inactive and thus allowing its delivery into cells intact, protected from nonspecific degradation within cells.

Irradiation at nm restored DNAzyme activity, thus allowing the temporal control over the sensing activity of the DNAzyme for metal ions. Photocaged DNAzymes as a general method for sensing metal ions in living cells. N2 - DNAzymes, which are sequences of DNA with catalytic activity, have been demonstrated as a potential platform for sensing a wide range of metal ions.

To overcome this limitation, a photoactivatable or photocaged DNAzyme was designed and synthesized, and its application in sensing ZnII in living cells was demonstrated. AB - DNAzymes, which are sequences of DNA with catalytic activity, have been demonstrated as a potential platform for sensing a wide range of metal ions. Abstract DNAzymes, which are sequences of DNA with catalytic activity, have been demonstrated as a potential platform for sensing a wide range of metal ions.

Catalytic DNA. Metal ions. Catalyst activity. Angewandte Chemie - International Edition , 53 50 , Angewandte Chemie - International Edition. In: Angewandte Chemie - International Edition. Access to Document Link to publication in Scopus. Link to citation list in Scopus.

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Photocaged DNAzymes as a General Method for Sensing Metal Ions in Living Cells

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